Antibody Response of Sheep and Goats in Saudi Arabia to the Live Attenuated Rift Valley Fever Vaccin


By Abdelhamid A.M. Elfadil, Shahta M. Musa, Sami S.M. Ali , Abdelhamid A. M. Elfadil, Shahta M. Musa 2, Sami S. M. Ali.


A field trial was performed on 30 female sheep and goats as a vaccination group and 33 males as a control to evaluate the immunogenicity of the live attenuated RVF vaccine (Smithburn strain). Immunoglobulin M (IgM) antibodies to RVF virus were detected in 10%, 3% and 10% of vaccinated animals in the first, second and third week following inoculation, respectively. Immunoglobulin G (IgG) antibodies were detected in 37%, 80% and 87% of vaccinated animals in the second, third and fourth week, respectively. Regarding detection of IgM and IgG antibodies, significant differences were observed between vaccinated and control animals (p-value = 0.005 and 0.0002 respectively). The results demonstrate a strong positive association between detection of IgG antibodies in the sera of vaccinated animals and vaccination (OR = 208, p-value = 0.0002). Both sheep and goats in the vaccination group responded to the vaccine. No significant difference was observed between vaccinated sheep and goats regarding detection of IgG antibodies in the second, third or fourth week following inoculation. Regarding detection of IgM antibodies, significant differences were observed between vaccinated sheep and goats (p-value = or < 0.005). The percent of IgM antibodies positive sheep was more than the percent of IgM positive goats in the three examinations.


  • 1- OIE Manual (1996). Rift Valley fever. Chapter [2. 1. 8, 102-108].
  • 2- Elfadil A. A., Musa S. M., Elkhamees M., Elmujalli D., El-Ahmed K. (2004). Epidemiologic study on Rift Valley fever in the South West of the Kingdom of Saudi Arabia. Journal of Science and Technology, 5 (1), 110-118.
  • 3- Barnard B. J. H. (1979). Rift Valley fever vaccine-antibody and immune response in cattle to live and an inactivated vaccine. Journal of south Africa Veterinary Association, 50, 155-157.
  • 4- Hubbard K. A., Baskerville A. and Stephenson J. R. (1991). Ability to mutagenised virus variant to protect young lambs from Rift Valley fever. American Journal of Veterinary Research, 52, 50-55.
  • 5- Meadors G. f., Gibbs P. H., and peters C. J. (1986). Evaluation of new Rift Valley fever vaccine: safety and immunogenicity trials. Vaccine, 4, 179-184.
  • 6- Morril J. C., Jennings G. B., Caplan H., Turrel M. J., Johnson A. J., and peters C. J. (1987). Pathogenicity and immunogenicity of a mutagenattenuated Rift Valley fever virus immungen in pregnant ewes American Journal of Veterinary Research, 48, 1042-1047.
  • 7- Paweska J. T., Burt F. J., Anthony F.,Smith S. J., Grobbelaar A. A., Croft J. E., Ksiazek T. J., Swanepoel R . (2003). IgG-sondwich and IgM-capture enzyme-linked immunosorbent assay for the detection of antibody to Rift Valley fever virus in domestic ruminants. Journal of Virological Methods, November 2003, 113 (2): 103-112.
  • 8- Madani T. A., Almazrou Y. Y., Aljafri M. H., et al. (2003). Rift Valley fever epidemic in Saudi Arabia: epidemiological, clinical, and laboratory characteristics. Journal of Clinical Infectious Diseases, 2003: 37, 1084-1092.
  • 9- Tomori O. (1979). Clinical, virological and serological response of the West African dwarf sheep to experimental infection with different strains of Rift Valley fever virus. Research in Veterinary Science, March 1979, 26(2): 152- 159.
  • 10- Losos G. J. (1986). Rift Valley fever vaccination: in Infectious Tropical Diseases of Domestic Animals, published in association with international Development Research Center, published in the USA by Churchill livingstone Inc. New York, 594-595.
  • 11- Saad S., Abdelsameea M. M. and Shalakamy E. (1997). Effect of attenuated Rift Valley fever vaccine on bulls semen. Proceeding 11, Fourth Scientific Congress, 7-9 December 1997, Assiut University, Assiut, Egypt, 168-171.